Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition

D. David Nowack; Dorothy M. Morré; D. James Morré

doi:10.1007/978-1-4684-5667-7_18

Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition

D. David Nowack, Dorothy M. Morré, D. James Morré

Purdue University

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised. Non-uniform homogenisation, contamination by organelles other than plasma membrane and changes in culture conditions, together with the transformation process itself, all tend to confound the fractionation of plasma membranes from cells and hepatomas.

Original language	American English
Title of host publication	Separations Using Aqueous Phase Systems
Subtitle of host publication	Applications in Cell Biology and Biotechnology
Place of Publication	New York, NY
Publisher	Plenum Press
Pages	109-110
ISBN (Electronic)	9781468456677
ISBN (Print)	9781468456691
DOIs	https://doi.org/10.1007/978-1-4684-5667-7_18
State	Published - 1989
Externally published	Yes

Disciplines

Biophysics

Access to Document

10.1007/978-1-4684-5667-7_18License: Unspecified

Cite this

Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition. / Nowack, D. David; Morré, Dorothy M.; Morré, D. James.
Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology. New York, NY: Plenum Press, 1989. p. 109-110.

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

@inbook{2c438704bf55436d86ba5df89f84079d,

title = "Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition",

abstract = "Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised. Non-uniform homogenisation, contamination by organelles other than plasma membrane and changes in culture conditions, together with the transformation process itself, all tend to confound the fractionation of plasma membranes from cells and hepatomas.",

author = "Nowack, \{D. David\} and Morr{\'e}, \{Dorothy M.\} and Morr{\'e}, \{D. James\}",

note = "Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised.",

year = "1989",

doi = "10.1007/978-1-4684-5667-7\_18",

language = "American English",

isbn = "9781468456691",

pages = "109--110",

booktitle = "Separations Using Aqueous Phase Systems",

publisher = "Plenum Press",

}

TY - CHAP

T1 - Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition

AU - Nowack, D. David

AU - Morré, Dorothy M.

AU - Morré, D. James

N1 - Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised.

PY - 1989

Y1 - 1989

N2 - Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised. Non-uniform homogenisation, contamination by organelles other than plasma membrane and changes in culture conditions, together with the transformation process itself, all tend to confound the fractionation of plasma membranes from cells and hepatomas.

AB - Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised. Non-uniform homogenisation, contamination by organelles other than plasma membrane and changes in culture conditions, together with the transformation process itself, all tend to confound the fractionation of plasma membranes from cells and hepatomas.

UR - https://link.springer.com/chapter/10.1007/978-1-4684-5667-7_18

U2 - 10.1007/978-1-4684-5667-7_18

DO - 10.1007/978-1-4684-5667-7_18

M3 - Chapter (peer-reviewed)

SN - 9781468456691

SP - 109

EP - 110

BT - Separations Using Aqueous Phase Systems

PB - Plenum Press

CY - New York, NY

ER -

Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition

Abstract

Disciplines

Access to Document

Other files and links

Cite this